Dr. Rafal P. Witek, an esteemed Key Opinion Leader (KOL) specialising in hepatocyte research, examines the factors influencing the performance of cryopreserved primary hepatocytes in in vitro cell culture. This includes a thorough analysis of selection criteria and sources of variability such as donor differences, process fluctuations, and functional stability. The webinar will delve into distinct criteria applicable to both 2D and 3D applications, empowering participants to effectively choose optimal primary hepatocyte lots for their respective studies. Moreover, attendees will gain insights into best practices for handling primary hepatocytes throughout storage, thawing, plating, and 3D aggregation, aimed at mitigating challenges associated with data reproducibility and variability.
